Journal Article

Molecular structure of human topoisomerase IIα revealed by atomic force microscopy

Saju R. Nettikadan, Christine S. Furbee, Mark T. Muller and Kunio Takeyasu

in Microscopy

Published on behalf of The Japanese Society of Microscopy

Volume 47, issue 6, pages 671-674
Published in print January 1998 | ISSN: 2050-5698
Published online January 1998 | e-ISSN: 2050-5701 | DOI: https://dx.doi.org/10.1093/oxfordjournals.jmicro.a023641
Molecular structure of human topoisomerase IIα revealed by atomic force microscopy

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The entire human topoisomerase II alpha (hTopoIIα) dimer was expressed in the yeast Saccaromyces cerevisiae, purified to homogeneity, and subjected to atomic force microscopy (AFM) under a tapping mode. Molecular images obtained exhibited a ‘heart or donut-like‘ structure with a large axial hole. The main benefit of the application of AFM to study the hTopoIIα is that clear images of the internal ‘pore’ have been achieved without crystallization, staining, or fixation of the sample. These images are consistent with the model in which topoisomerase II has a large internal gate for DNA strand passage.

Keywords: topoisomerase II (human); atomic force microscopy; axial pore

Journal Article.  0 words. 

Subjects: Biological Sciences

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