Journal Article

Chronic effects of endothelin-3 on blood pressure and renal haemodynamics in rats

F. Martinez, G. Deray, M. Dubois, C. Jacquiaud, F. Schmitt and C. Jacobs

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 11, issue 2, pages 270-274
Published in print February 1996 | ISSN: 0931-0509
Published online February 1996 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/oxfordjournals.ndt.a027252
Chronic effects of endothelin-3 on blood pressure and renal haemodynamics in rats

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Background.

Renal vasoconstriction and systemic hypertension are well-known effects of bolus or short-term endothelin administration. However, the role of endothelin as a circulating hormone remains largely unknown.

Methods.

The present study explores the effects of endothelin-3 (ET-3) on renal haemodynamics and systemic blood pressure during a 3-h and a 3-day intravenous infusion in rats. Male Sprague-Dawley (SD) rats were infused with vehicle (group 1) or ET-3 (group 2, 10 ng/kg per min; group 3, 50 ng/kg per min) delivered via osmotic minipumps into the right jugular vein for 3 days. On day 3 after pump implantation, rats were anaesthetized with Inactin and surgically prepared for assessment of mean arterial blood pressure (MABP), renal plasma flow (RPF), glomerular filtration rate (GFR), and renal vascular resistance (RVR). The same parameters were assessed during a 3-h ET-3 infusion study in SD rats (group 4, vehicle; group 5, ET-3, 10 ng/kg per min; group 6, ET-3, 50 ng/kg per min).

Results.

In 3-day infused rats, ET-3 induced a significant decrease in RPF (−22±7% and −26±8% for group 2 and group 3 respectively, P<0.05 vs group 1) and an increase in RVR (+40±11% for groups 2 and 3; P<0.05 vs group 1); 50 ng/kg per min ET-3 significantly decreased GFR (−17%, P<0.05 vs group 1). MABP was not significantly affected by endothelin infusion. In acute infusion studies a decrease of the same magnitude was seen for the renal haemodynamics values. 50 ng/kg per min ET-3 increased MABP; a systemic effect that disappeared after the 3-day infusion.

Conclusions.

This study suggests that intravenously administered ET-3 in the rat has only a transient effect on systemic blood pressure, whereas it induces alterations in renal haemodynamics after both acute and chronic perfusions.

Keywords: blood pressure; endothelin-3; experimental; natriuresis; osmotic pump; renal haemodynamics

Journal Article.  0 words. 

Subjects: Nephrology

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