Journal Article

Increased mononuclear cell membrane fluidity and increased B lymphocyte HLA class I expression in IgA nephropathy

T. R. Ringrose, T. H. Thomas and R. Wilkinson

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 11, issue 2, pages 287-292
Published in print February 1996 | ISSN: 0931-0509
Published online February 1996 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/oxfordjournals.ndt.a027255
Increased mononuclear cell membrane fluidity and increased B lymphocyte HLA class I expression in IgA nephropathy

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Background.

The functions of membrane bound proteins are regulated by the physical properties of the cell membrane. Lymphocyte dysfunction in IgA nephropathy may therefore be related to abnormal cell membrane fluidity. In this study we have examined peripheral blood mononuclear cell membrane fluidity and the expression of HLA antigens on the surface of lymphocytes in IgA nephropathy subjects compared to normal controls and disease controls.

Methods.

Twenty-two IgA nephropathy subjects with normal or mildly elevated serum creatinine (serum creatinine <130 μmol/l), 21 normal control subjects, and 16 disease control subjects were studied. Fluorescence anisotropy was determined using diphenylhexatriene, which partitions deep into the cell membrane, and trimethylammonium-DPH, which partitions more superficially. The lymphocyte expression of HLA antigens was measured by flow cytometry.

Results.

Fluorescence anisotropy for diphenylhexatriene of mononuclear cells from IgA nephropathy subjects was significantly lower compared to mononuclear cells from normal control subjects and disease control subjects, indicating higher membrane fluidity (median values, IgA nephropathy 0.161; normal control 0.175; disease control 0.175; P<0.001 and P<0.001). Fluorescence anisotropy for trimethylammonium-DPH was lower in the IgA nephropathy group compared to the normal control group (median values, IgA nephropathy 0.268; normal control 0.274; P<0.001), but not significantly different compared to the disease control group (0.272). HLA class I expression on the surface of B lymphocytes was significantly higher in the IgA nephropathy group compared to the normal control and disease control groups (median values, IgA nephropathy 30364, normal control 15495; disease control 16907; P=0.0001 and P=0.002 respectively).

Conclusions.

This study provides evidence of abnormal cell membrane architecture and increased HLA class I expression in IgA nephropathy.

Keywords: fluorescence anisotropy; HLA expression; IgA nephropathy; lymphocyte; membrane fluidity

Journal Article.  0 words. 

Subjects: Nephrology

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