Journal Article

Daily short exposure of cultured mesothelial cells to lactated, high-glucose, low-pH peritoneal dialysis fluid induces a low-profile regenerative steady state

A. Shostak, K. Pivnik and L. Gotloib

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 11, issue 4, pages 608-613
Published in print April 1996 | ISSN: 0931-0509
e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/oxfordjournals.ndt.a027348
Daily short exposure of cultured mesothelial cells to lactated, high-glucose, low-pH peritoneal dialysis fluid induces a low-profile regenerative steady state

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This study was designed to evaluate cytotoxic effects and influence upon cell growth of cultured mesothelial cells exposed to modified 4.25% Dianeal dialysate fluid (M-199 in Dianeal solution, glucose 4.25 g, pH 5.2) (Expr. group), 60 min a day for a total period of follow-up of 13 consecutive days, compared with that observed in a control group (C).

Beginning on day 7, the cell counts in group C were significantly higher than those observed at zero time (P<0.05). Cell counts in the experimental group showed no significant differences between the first day of culture and each one of the 13 consecutive days of follow-up.

Thymidine incorporation into DNA observed on the first day in C, was significantly higher (P<0.01) beginning on the 10th day. Values observed in the experimental group were low during the whole period of follow-up. LDH mean values at each time interval, were significantly higher (P<0.01 and <0.001) for cells exposed to the dialysis solution.

Repeated exposure of the mesothelium to 40 mMol/l lactate and high glucose concentrations induced severe cell injury and death, decreased cell growth and, consequently, a reduced rate of regeneration which is extended as long as the repeated exposure is maintained.

Keywords: biocompatibility of dialysis solutions; mesothelial cells culture

Journal Article.  0 words. 

Subjects: Nephrology

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