Journal Article

The Seed-Specific Transcription Factor VP1 (OSVP1) Is Expressed in Rice Suspension-Cultured Cells

Hitoshi Nakagawa, Eiko Ohkura, Kunio Ohmiya and Tsukaho Hattori

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 37, issue 3, pages 355-362
Published in print April 1996 | ISSN: 0032-0781
e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a028953
The Seed-Specific Transcription Factor VP1 (OSVP1) Is Expressed in Rice Suspension-Cultured Cells

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

A seed-specific transcriptional regulator, VP1, is required for the induction of ABA-regulated genes that include Lea (late embryogenesis abundant protein) genes. Although the induction of one rice Lea gene, Osem, by ABA is normally restricted to seed tissues, we found that the expression was strongly induced by ABA in the Oc line of rice suspension-cultured cells. Since this observation suggested that rice VP1 (OSVP1) protein or a functionally similar protein might be expressed in the cultured cells, we analyzed the expression of Osvpl in these cells at both the mRNA and the protein level, we detected Osvp1 mRNA and OSVP1 protein in the cultured cells at levels similar to or higher than those in developing embryos. In the cultured cells, neither the level of total cellular OSVP1 nor that of nuclear OSVP1 protein was affected by ABA. Based on the results, the mechanism for the transcriptional regulation of VP1-dependent ABA-inducible genes is discussed.

Keywords: AB13; Abscisic acid; Oryza sativa; Osvp1; Transcriptional regulator; viviparous-1

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.