Journal Article

A Step in the Biosynthesis of Gibberellins that Is Controlled by the Mutation in the Semi-Dwarf Rice Cultivar Tan-Ginbozu

Shoko Ogawa, Tomonobu Toyomasu, Hisakazu Yamane, Noboru Murofushi, Ryoichi Ikeda, Yasuyuki Morimoto, Yasuhiko Nishimura and Toshio Omori

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 37, issue 3, pages 363-368
Published in print April 1996 | ISSN: 0032-0781
e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a028954
A Step in the Biosynthesis of Gibberellins that Is Controlled by the Mutation in the Semi-Dwarf Rice Cultivar Tan-Ginbozu

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Genetic analysis and a comparison of endogenous levels of gibberellins between the semi-dwarf rice cultivar Tan-ginbozu and the corresponding normal cultivar Ginbozu have confirmed that Tan-ginbozu is a gibberellin deficient mutant and that the semi-dwarfism of Tan-ginbozu is controlled by a single recessive gene. A step in the biosynthesis of gibberellins that is blocked by the mutation in Tan-ginbozu had been considered to be the synthesis of ent-kaurene or an earlier step. However, the rate of production of ent-kaurene by Tan-ginbozu was almost the same as that by Ginbozu. By contrast, accumulation of only a small amount of ent-kaurene was detected in Tan-ginbozu, and the amount that accumulated was similar to that in Ginbozu that had been treated with 6.9 × 10-8 M uniconazole-P (an effective inhibitor of three oxidative steps in the pathway from ent-kaurene to ent-kaurenoic acid via entkaurenol and ent-kaurenal). The height of the treated Ginbozu plants was reduced to the same as that of Tanginbozu plants. Resembling Tan-ginbozu plants, Ginbozu plants that had been treated with uniconazole-P responded well to ent-kaurenoic acid and slightly to ent-kaurene and ent-kaurenol. Since the growth-promoting activity of enf-kaurenal in Tan-ginbozu was similar to that of ent-kaurene, our results suggest that the mutation in Tan-ginbozu blocks the three oxidative steps whereby ent-kaurene is converted to ent-kaurenoic acid.

Keywords: Dwarfism; ent-Kaurene; Gas chromatography/selected ion monitoring; Gibberellin-deficient mutant; Oryza sativa L. cv. Tan-ginbozu; Uniconazole-P

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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