Journal Article

Molecular Cloning and Characterization of a cDNA Clone That Encodes a Cdc2 Homolog from <i>Nicotiana tabacum</i>

Yulius Yulianto Setiady, Masami Sekine, Norimitsu Hariguchi, Hiroshi Kouchi and Atsuhiko Shinmyo

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 37, issue 3, pages 369-376
Published in print April 1996 | ISSN: 0032-0781
e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a028955
Molecular Cloning and Characterization of a cDNA Clone That Encodes a Cdc2 Homolog from Nicotiana tabacum

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We have isolated a cDNA clone (cdc2Nt1) that encodes a homolog of p34cdc2/CDC28 kinase from tobacco (Nicotiana tabacum). The cdc2Ntl protein showed extensive similarity to other homologs of Cdc2 from plants. Complementation studies showed that the cdc2Ntl gene was able to overcome cell cycle arrest at both the G1/S and the G2/M transitions of cdc28ts mutants of budding yeast, demonstrating that the cdc2Ntl protein was able to replace the Cdc28 kinase at both the G1/S and the G2/M transitions. Analysis of gene expression demonstrated that the cdc2Ntl gene was transcribed constitutively throughout the cell cycle but that it was preferentially expressed in actively dividing tobacco BY-2 cells.

Keywords: Cdc2 homolog; cdc28ts yeast mutants; cdc2Nt1; Nicotiana tabacum (tobacco); Proliferativestate-dependent expression

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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