Journal Article

Indolepyruvate Pathway for Indole-3-Acetic Acid Biosynthesis in <i>Bradyrhizobium elkanii</i>

Kiwamu Minamisawa, Ken-Ichiro Ogawa, Hideyuki Fukuhara and Jinichiro Koga

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 37, issue 4, pages 449-453
Published in print June 1996 | ISSN: 0032-0781
e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a028966
Indolepyruvate Pathway for Indole-3-Acetic Acid Biosynthesis in Bradyrhizobium elkanii

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Indole-3-acetaldehyde (IAAId) was detected in the culture supernatant of Bradyrhizobium elkanii. Deuteriumlabelled L-tryptophan (Trp) was incorporated into IAAId and indole-3-acetic acid (IAA), suggesting that B. elkanii produces IAA via IAAId from Trp. In B. elkanii cell suspension, indole-3-pyruvic acid (IPyA) was converted to IAAId, and exogenously added IAAId was rapidly converted to IAA. Furthermore, the activity of indolepyruvate decarboxylase (IPDC), which catalyzes the decarboxylation of IPyA to produce IAAId and is a key enzyme for IPyA pathway, was detected in B. elkanii cell-free extract. The IPDC activity depended on Mg2+ and thiamine pyrophosphate, cofactors of decarboxylation. This mounting evidence strongly suggests that IAA synthesis occurs via IPyA pathway (Trp → IPyA →p IAAId → IAA) in B. elkanii.

Keywords: Bradyrhizobium elkanii; Indole-3-acetic acid (IAA); Indole-3-acetaldehyde; Indolepyruvate decarboxylase

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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