Journal Article

Pollen of a Male-Sterile Mutant of <i>Arabidopsis thaliana</i> Isolated from a T-DNA Insertion Pool is Not Effectively Released from The Anther Locule

Soon Ki Park, Yong Hwi Yoon, Byung Chul Kim, Young Hyun Hwang, Il Kyung Chung, Hong Gil Nam and Dal Ung Kim

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 37, issue 5, pages 580-585
Published in print July 1996 | ISSN: 0032-0781
e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a028984
Pollen of a Male-Sterile Mutant of Arabidopsis thaliana Isolated from a T-DNA Insertion Pool is Not Effectively Released from The Anther Locule

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

We have isolated a male-sterile mutant from a pool of T-DNA insertional lines of Arabidopsis thaliana generated by an in planta transformation procedure [Chang et al. (1994) Plant J. 5: 551]. Pollen in this mutant is not effectively released from anther locules after cleavage of the stomium. Most mutant pollen grains are round, in contrast to the tricolpate wild-type pollen, and some pollen grains show an abnormal surface structure. Manually released mutant pollen grains are not fertile and show defects in pollen tube germination in vitro. Genetic analysis disclosed that this lesion is due to a single recessive nuclear mutation located on chromosome 3 closely linked to the gl1 locus. The mutation locus is tightly linked to the inserted T-DNA.

Keywords: Arabidopsis thaliana; In planta transformation; Male-sterile mutant; porl-1 mutation; T-DNA; insertional mutagenesis

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.