Journal Article

cDNA Cloning of a Wounding-Inducible Gene Encoding a Plastid ω-3 Fatty Acid Desaturase from Tobacco

Tatsurou Hamada, Takumi Nishiuchi, Hiroaki Kodama, Mitsuo Nishimura and Koh Iba

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 37, issue 5, pages 606-611
Published in print July 1996 | ISSN: 0032-0781
e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a028988
cDNA Cloning of a Wounding-Inducible Gene Encoding a Plastid ω-3 Fatty Acid Desaturase from Tobacco

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A cDNA encoding the plastid ω-3 fatty acid desaturase was isolated from a tobacco (Nicotiana tabacum cv. SR1) leaf cDNA library. The amino terminal extension of the deduced amino acid sequence of this clone had a characteristic feature of the transit peptides of plastid-destined proteins. Northern analysis indicated that the mRNA corresponding to this cDNA was present in leaves, but was not detected in roots. Responses to wounding of the plastid and microsome ω-3 desaturase genes were investigated in tobacco leaves. The mRNA level of the plastid ω-3 desaturase gene increased to about 2-fold that of unwounded controls at 12 h after a wounding treatment. On the other hand, the mRNA level of the microsome ω-3 desaturase gene remained constant in the wounded leaves. Linolenic acid contents of major leaf polar lipids increased by wounding. These results indicate that wounding enhances the accumulation of the plastid ω-3 desaturase mRNA, and increases the conversion of linoleic acid to linolenic acid in leaf polar lipids.

Keywords: Linolenic acid; Nicotiana tabacum; Plastid; Wounding; ω-3 fatty acid desaturase

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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