Journal Article

Cell-Cell Recognition during Fertilization in a Red Alga, <i>Antithamnion sparsum</i> (Ceramiaceae, Rhodophyta)

Gwang Hoon Kim, In Kyu Lee and Lawrence Fritz

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 37, issue 5, pages 621-628
Published in print July 1996 | ISSN: 0032-0781
e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a028990
Cell-Cell Recognition during Fertilization in a Red Alga, Antithamnion sparsum (Ceramiaceae, Rhodophyta)

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A gamete recognition mechanism in Antithamnion sparsum Tokida is proposed based on experiments using various lectins and carbohydrates. Spermatial binding to trichogynes is inhibited by pre-incubation of spermatia with concanavalin A (ConA) and/or L-fucose, while trichogyne receptors are blocked by the complementary carbohydrate α-methyl D-mannose and/or the lectin Ulex europaeus agglutinin (UeA1). Binding inhibition (40–50%) was observed with 10–50 mM carbohydrates and 25–50 μg ml-1 lectins. The inhibitory effects of ConA and UeA1 is partially reversed (to 80–90% of controls) by addition of α-methyl D-mannose and L-fucose, respectively. Lectin binding to spermatial surfaces was visualized by Fluorescein isothiocyanate (FITC) conjugated ConA, whereas carbohydrate receptors along the trichogyne and spermatium were localized with α-mannosylated-FITC-albumin and L-fucosylated-FITC-albumin, respectively. These results suggest that gamete recognition in Antithamnion sparsum is mediated by a double-docking recognition system consisting of spermatia possessing surface L-fucose receptors and α-methyl D-mannose moiety, and trichogynes possessing the complementary receptors.

Keywords: Antithamnion sparsum; Carbohydrate receptor; Cell-cell recognition; Fertilization; Lectin; Rhodophyta

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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