Journal Article

Two Plasma Membrane H<sup>+</sup>-ATPase Genes Expressed in Guard Cells of <i>Vicia faba</i> Are Also Expressed Throughout the Plant

Audrey E. Hentzen, Lawrence B. Smart, Larry E. Whimmers, Henry H. Fang, Julian I. Schroeder and Alan B. Bennett

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 37, issue 5, pages 650-659
Published in print July 1996 | ISSN: 0032-0781
e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a028994
Two Plasma Membrane H+-ATPase Genes Expressed in Guard Cells of Vicia faba Are Also Expressed Throughout the Plant

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  • Molecular and Cell Biology
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Guard cells modulate stomatal apertures in response to hormones, metabolic demands and environmental stimuli. The guard cell PM H+-ATPases play a critical role in this process by generating the electrochemical gradient to drive solute transport and concomitant water flux. The PM H+-ATPase activity is specifically regulated by red and blue light, fungal toxins and auxin. To determine if the unique responsiveness of the guard cell PM H+-ATPase is due to the expression of a cell-specific isoform, we amplified by PCR, and cloned portions of PM H+-ATPase genes VHA1 and VHA2, which are expressed in guard cell protoplasts (GCP). In situ hybridization to leaf tissue sections indicated that VHA1 and VHA2 genes were expressed in guard cells and mesophyll cells but not in epidermal cells or vascular tissues. Furthermore, a gene-specific quantitative reverse transcription (RT)-PCR detected VHA1 and VHA2 mRNAs in both GCP and mesophyll cell protoplast mRNA as well as in mRNA isolated from roots, leaves, stems and flowers. Thus, two PM H+-ATPase genes expressed in guard cells are also expressed in many other tissues and cell types. This suggests that the unique responsiveness of the guard cell PM H+-ATPases to environmental stimuli results from cell-specific signal transduction pathways rather than the expression of a cell-specific PM H+-ATPase.

Keywords: Guard cell; H+-ATPase; Vicia faba; PCR (polymerase chain reaction)

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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