Journal Article

In Vitro Phosphorylation of Proteins in IAA-Treated Mesocotyls in <i>Zea mays</i>

Ryoichi Kato, Sachiko Takatsuna, Tsuyoshi Wada, Yumi Narihara and Takashi Suzuki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 37, issue 5, pages 667-672
Published in print July 1996 | ISSN: 0032-0781
e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a028996
In Vitro Phosphorylation of Proteins in IAA-Treated Mesocotyls in Zea mays

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Five-mm sections of elongation zones of Zea mesocotyls were incubated for designated periods with various concentrations of IAA. In vitro protein phosphorylation in the soluble fraction (85,000 × g supernatant) prepared from the sections was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The phosphorylation of proteins in the soluble fraction that had been prepared from sections incubated for 20 min in the presence of 10˜s M IAA was greater than that in the sections incubated for 20 min without IAA. The amount of phosphorylation of proteins per protein became higher when higher concentrations increased (10˜8—10˜5M). The growth of sections incubated in the presence of 10˜8 M IAA or higher concentrations was greater than that of sections incubated in the absence of IAA. The promotion of growth by IAA was greater at higher concentrations of IAA. Proteins in the soluble fraction, prepared from sections incubated for 20 min in the presence of 10˜5 M IAA, were phosphorylated in the presence of either 10 fM cAMP, 10 μM cGMP, 100 μM W-7, 100 μM W-5, 20 μM H-7 or 20 μM HA1004. The calmodulin antagonist, W-7, and the inhibitor of protein kinase C, H-7, inhibited the phosphorylation of proteins stimulated by incubation with IAA. These results suggest that IAA promotes cell elongation via protein phosphorylation that depends on calmodulin-dependent protein kinase and protein kinase C.

Keywords: IAA treatment; Increase of growth; Protein phosphorylation; Zea mesocotyl

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.