Journal Article

Molecular and Biochemical Characterization of Three Anthocyanin Synthetic Enzymes from <i>Gentiana triflora</i>

Yoshikazu Tanaka, Keiko Yonekura, Masako Fukuchi-Mizutani, Yuko Fukui, Hiroyuki Fujiwara, Toshihiko Ashikari and Takaaki Kusumi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 37, issue 5, pages 711-716
Published in print July 1996 | ISSN: 0032-0781
e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029004
Molecular and Biochemical Characterization of Three Anthocyanin Synthetic Enzymes from Gentiana triflora

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Full length cDNA clones of flavonoid 3′,5′-hydroxylase, dihydroflavonol 4-reductase and flavonoid 3-glucosyltransferase were cloned from petals of Gentiana triflora. Their sequences were homologous to counterparts from other plants. Flavonoid 3′,5′-hydroxylase and flavonoid 3-glucosyltransferase were enzymatically characterized by expressing cDNAs in heterologous expression systems.

Keywords: Anthocyanin; Cloning; Flavonoid; Gene expression; Gentian {Gentiana triflora)

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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