Journal Article

Uptake of Neutral Red by the Vacuoles of a Green Alga, <i>Micrasterias pinnatifida</i>

Megumi Ehara, Tetsuko Noguchi and Katsumi Ueda

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 37, issue 6, pages 734-741
Published in print September 1996 | ISSN: 0032-0781
e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029007
Uptake of Neutral Red by the Vacuoles of a Green Alga, Micrasterias pinnatifida

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Neutral red (NR) in the culture medium entered the vacuoles of a green alga, Micrasterias pinnatifida, at a higher rate at pH 8 than at pH 5. NR remained soluble in vacuoles of cells cultured at pH 5, while it precipitated and formed granules in cells cultured at pH 8. The vacuoles of cells cultured at pH 8 contained fibrils, but those of cells cultured at pH 5 did not. The amount of NR that entered the cells was markedly reduced by the addition to the medium of nigericin at 10-5M, monensin at 10-5M, bafilo-mycin A1 at 10-5M, or ammonium chloride at 50 mM. The formation of NR granules in vacuoles were strongly inhibited and the disorganization of NR granules were accelerated by the addition of nigericin at 10-5M, or bafilomycin A1 at 10-5M to the culture medium. The possibility is discussed that NR which enters vacuoles might become positively charged (NRH+) by protons brought into vacuoles by proton pumps and that NRH+ might combine with some negatively charged macromolecules to form aggregates or granules.

Keywords: Membrane transport; Micrasterias pinnatifida; Neutral red; Storage; Vacuole

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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