Journal Article

Phosphorylation of a Protein (pp56) is Related to the Regeneration of Rice Cultured Suspension Cells

Setsuko Komatsu, Taizo Masuda and Keisuke Abe

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 37, issue 6, pages 748-753
Published in print September 1996 | ISSN: 0032-0781
e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029009
Phosphorylation of a Protein (pp56) is Related to the Regeneration of Rice Cultured Suspension Cells

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Short-term cultured suspension cells of rice (Oryza sativa L.) are capable of regeneration, but not in long-term culture. For clarification of the mechanism of regeneration, protein phosphorylation in short-term and long-term cultured suspension cells was compared by two dimensional- polyacrylamide gel electrophoresis. A 56 kDa protein having an isoelectric point of 4.5 was phosphorylated in vitro in short-term cultured suspension cells, but was not phosphorylated after regeneration. This protein in longterm cultured suspension cells remained phosphorylated after transfer to the regeneration medium. However, using an antibody raised against this protein from short-term cultured suspension cells, it was always detected in long-term and short-term cultured suspension cells after transfer to the regeneration medium. The partial amino acid sequence of the HPLC-purified protein showed homology to a calcium-binding protein from maize. The phosphorylation of the 56 kDa protein (pp56) appears to be associated with the regeneration of cultured rice cells.

Keywords: Cultured cells; Protein phosphorylation; Regeneration; Rice

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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