Journal Article

Structure and Expression of Mitochondrial Citrate Synthases from Higher Plants

Ursula La Cognata, Volker Landschütze, Lothar Willmitzer and Bernd Müller-Röber

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 37, issue 7, pages 1022-1029
Published in print October 1996 | ISSN: 0032-0781
e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/oxfordjournals.pcp.a029033
Structure and Expression of Mitochondrial Citrate Synthases from Higher Plants

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  • Molecular and Cell Biology
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Mitochondrial citrate synthase (EC 4.1.3.7) represents the first enzyme of the tricarboxylic acid cycle, catalyzing the condensation of acetyl-CoA and oxaloacetate, finally yielding citrate and CoA. We report here the isolation of cDNA clones encoding citrate synthase from Nicotiana tabacum, Beta vulgaris and Populus. Nucleotide and deduced amino acid sequences were compared with previously published sequences of mitochondrial citrate synthases from Arabidopsis thaliana and potato, as well as with the sequence of glyoxysomal citrate synthase from pumpkin. Homologies between the various plant mitochondrial enzymes were in the range from 77.2% (potato vs. Arabidopsis) to 94.2% (potato vs. tobacco) on the nucleotide level (coding regions only), and in the range from 70.1% to 90.4% (potato vs. Arabidopsis, and potato vs. tobacco, respectively) on the amino acid level. Identities of the mitochondrial isozymes to the pumpkin glyoxysomal enzyme were below 30% on the nucleotide and amino acid level. In Northern blot experiments citrate synthase mRNA was detected in all tissues analyzed. However, levels of expression showed tissue dependency despite the fact that citrate synthase is usually considered a house-keeping enzyme. Whether these different levels of expression reflect tissuespecifc variations with respect to basic metabolism awaits further analysis.

Keywords: Beta vulgaris; cDNA cloning; Citrate synthase (EC 4.1.3.7); Expression analysis; Nicotiana tabacum; Populus sp

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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