Journal Article

Mechanism of Electron Flow through the QB Site in Photosystem II. 4. Reaction Mechanism of Plastoquinone Derivatives at the QB Site in Spinach Photosystem II Membrane Fragments

Hiroyuki Koike, Koichi Yoneyama, Yasuhiro Kashino and Kazuhiko Satoh

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 37, issue 7, pages 983-988
Published in print October 1996 | ISSN: 0032-0781
e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029048
Mechanism of Electron Flow through the QB Site in Photosystem II. 4. Reaction Mechanism of Plastoquinone Derivatives at the QB Site in Spinach Photosystem II Membrane Fragments

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Interactions of externally added plastoquinone (PQ) derivatives (PQ0-PQ3) with the photosystem II (PSII) acceptor side were investigated in PSII membrane fragments prepared from spinach by measuring the photoreduction rates of PQ derivatives at various PQ concentrations, and the following results were obtained.

From the kinetic analysis, all the PQ derivatives (PQ0-PQ3) except PQ3 were shown to accept electrons at two sites (the QB site and the PQ site) as in the case of Synechococcus vulcanus PSII particles with benzoquinone derivatives [Satoh et al. (1995) Plant Cell Physiol. 36: 597].

Affinities of PQ derivatives at the QB site increased as the length of the isoprene side chain got longer, while those at the PQ site were not very much different for all the PQ derivatives tested in this study.

The inhibitory effect of DCMU was noncompetitive, and, therefore, the affinity of PQ3 for the PQ site was determined while that for the QB site could not be estimated presumably due to its fairly high affinity to the site.

Based on the results obtained using PQ derivatives, the mechanism of interaction of an authentic PQ, PQ9, at the QB site is discussed.

Keywords: Photosynthesis; Photosystem II; Plastoquinone; QB site; Spinach

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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