Journal Article

Tissue-Specific Expression Conferred by the <i>S</i>-Adenosyl-L-Methionine Synthetase Promoter of <i>Arabidopsis thaliana</i> in Transgenic Poplar

Kristine Vander Mijnsbrugge, Marc Van Montagu, Dirk Inzé and Wout Boerjan

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 37, issue 8, pages 1108-1115
Published in print December 1996 | ISSN: 0032-0781
e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/oxfordjournals.pcp.a029061
Tissue-Specific Expression Conferred by the S-Adenosyl-L-Methionine Synthetase Promoter of Arabidopsis thaliana in Transgenic Poplar

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

In Arabidopsis the promoter of the gene encoding S-adenosyl-L-methionine synthetase (SAM-S) Psam-1 confers expression preferentially in the vascular tissue. In search for promoters that drive expression in particular cells of the lignifying tissues in trees, we have analyzed the expression pattern conferred by the Psam-1 promoter in transgenic poplar. Histochemical analyses demonstrated rβ glucuronidase (GUS) activity mainly in phloem and cortex tissue throughout the plant, and in root tips. Fluorimetric assays showed high GUS activity in the tissues outside (phloem, cortex and cork) compared to those inside (xylem and pith) of the cambial layer. In contrast, the endogenous SAM-S activity was high in tissues inside and low in tissues outside of the cambial layer. RNA gel blot analysis demonstrated a high transcript level of the endogenous sam-s gene(s) in tissues both outside and inside the cambial layer. This indicates that the low SAM-S activity in the bark was at least partially due to translational and/or post-translational regulation of the endogenous sam-s gene(s). In dormant transgenics, the tissue specificity was conserved, but the activity levels were up to 10-fold reduced.

Keywords: S-adenosyl-L-methionine synthetase; Cinnamyl alcohol dehydrogenase; rβ-Glucuronidase; Populus tremula × P. alba; Translational and/or post-translational regulation

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.