Journal Article

Premature Drying Increases the GA-Responsiveness of Developing Aleurone Layers of Barley (<i>Hordeum vulgare</i> L.) Grain

Liwen Jiang, Allison R. Kermode and Russell L. Jones

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 37, issue 8, pages 1116-1125
Published in print December 1996 | ISSN: 0032-0781
e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029062
Premature Drying Increases the GA-Responsiveness of Developing Aleurone Layers of Barley (Hordeum vulgare L.) Grain

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The effect of premature drying on the sensitivity of aleurone layer cells of developing barley (Hordeum vulgare L.) grain to gibberellic acid (GA3) was investigated. The capacity of barley aleurone layer cells to respond to GA3, as indicated by α-amylase synthesis and secretion by de-embryonated grain, increased during the later stages of development. Aleurone layers of immature grain (younger than 30 d after anthesis; DAA) were not capable of producing αamylase in response to GA3; however, premature drying at this stage promoted GA-responsiveness resulting in the induction of mRNA for α-amylase and increased α-amylase synthesis and secretion. Preincubation of the immature grain or its maintenance at 100% relative humidity prior to exposure of the de-embryonated grain to GA3 also led to an enhanced capacity of the aleurone layer to produce αamylase and its mRNA as compared to the fresh, untreated grain. However, the amount of mRNA and enzyme produced was much lower than that induced by premature drying. Moreover, following these nondrying treatments, the aleurone layer cells remained unresponsive to exogenous GA3; the same amount of enzyme was produced in the absence of applied GA3. Transient expression of chimeric gene constructs in aleurone layer cells of de-embryonated grain suggest that drying up-regulates the α-amylase gene promoter in response to GA3. We conclude that desiccation is required for barley aleurone layer cells to become responsive to GA3 and hence express their full potential for αamylase synthesis and secretion.

Keywords: α-Amylase; Barley; Desiccation; Development; GA sensitivity

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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