Journal Article

Preparation of a Monoclonal Antibody against Soybean Nodule Uricase (nod-35), and Immunoblot Analysis of the Expression of nod-35 in Tissues of Various Legumes

Shigeyuki Tajima, Kenichi Takane, Kyoko Ohkawa, Akio Sugimoto and Katsuichiro Okazaki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 37, issue 8, pages 1188-1192
Published in print December 1996 | ISSN: 0032-0781
e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029071
Preparation of a Monoclonal Antibody against Soybean Nodule Uricase (nod-35), and Immunoblot Analysis of the Expression of nod-35 in Tissues of Various Legumes

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Immunoblot analysis showed that uricases in nonureide-transporting determinate nodules (Canavalia gladiata and Lotus japonicus) did not react with a monoclonal antibody against soybean nodule uricase, suggesting different immunological reactivities from those of uricases of ureide-transporting legumes.

Keywords: Legumes; Monoclonal antibody; Nodules; Uricase (EC 1.7.3.3)

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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