Journal Article

Phosphoenolpyruvate Carboxylase of Maize Leaves: an Improved Method for Purification and Reduction of the Inhibitory Effect of Malate by Ethylene Glycol and Bicarbonate

Noriyuki Ogawa, Takako Kai, Naohiro Yabuta and Katsura Izui

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 1, pages 76-80
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029088
Phosphoenolpyruvate Carboxylase of Maize Leaves: an Improved Method for Purification and Reduction of the Inhibitory Effect of Malate by Ethylene Glycol and Bicarbonate

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Light-adapted and dark-adapted forms of phosphoenolpyruvate carboxylase were purified from maize leaves by an improved method that included chromatography on Butyl-Toyopearl in the presence of ethylene glycol. The inhibition by malate was relieved not only by increasing concentrations of ethylene glycol but also by bicarbonate at pH 7.0.

Keywords: C4photosynthesis; Ethylene glycol; Malate inhibition; Phosphoenolpyruvate carboxylase (EC 4.1.1.31); Purification; Zea mays

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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