Journal Article

Pectin Depolymerase Activities Associated with Cell Walls from <i>Cicer arietinum</i> L. Epicotyl

Jos´ Manuel Sevillano, Ignacio Zarra and Jose Luis Acebes

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 11, pages 1259-1263
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029113
Pectin Depolymerase Activities Associated with Cell Walls from Cicer arietinum L. Epicotyl

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The hydrolytic activity of the proteins extracted with 3 M LiCl from chick-pea (Cicer arietinum) cell walls to pec-tic fractions extracted with 50 mM trans-l,2-diaminocy-clohexane-N,N,N,N,-tetraacetic acid (CDTA) and 50 mM sodium carbonate was studied. The pectic fractions contained acidic polysaccharides with high molecular mass (higher than 5 × 103 kDa), mainly composed of uronic acids, galac-tose, arabinose and rhamnose. The extracted proteins depo-lymerized the pectic polysaccharides and also a commercial preparation of polygalacturonic acid from citrus, detected by a decrease in their viscosity and a shift of their molecular mass distribution. The extract was able to depolymerize a uronic acid-rich component in all the cases, although in different extent. Also, with regard to the CDTA-soluble pectins, a degradation of polyuronide and a shift of the molecular mass distribution of arabinogalactan was observed.

Keywords: Arabinogalactan; Cell wall; Cicer arietinum; Pectin; Pectin-depolymerase; Polyuronide

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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