Journal Article

Electron Flow from NAD(P)H Dehydrogenase to Photosystem I is Required for Adaptation to Salt Shock in the Cyanobacterium <i>Synechocystis</i> sp. PCC 6803

Yoshito Tanaka, Seiichi Katada, Hiroshi Ishikawa, Teruo Ogawa and Teruhiro Takabe

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 12, pages 1311-1318
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029123
Electron Flow from NAD(P)H Dehydrogenase to Photosystem I is Required for Adaptation to Salt Shock in the Cyanobacterium Synechocystis sp. PCC 6803

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The role of the NAD(P)H-dehydrogenase complex in adaptation to salt stress was examined in an ndhB-inacti-vated mutant of the cyanobacterium Synechocystis sp. PCC 6803. Wild-type cells and ndhB-inactivated mutant cells grew at similar rates under conditions of low salinity (<0.6M NaCl) and high CO2 (3%). However, when the concentration of NaCl in the culture medium was higher than 0.6 M, the mutant cells grew much more slowly than the wild-type cells. Upon addition of high concentrations of NaCl, the oxygen-evolving activity was rapidly inhibited but then it recovered, with the rate of recovery depending on the concentration of NaCl. The recovery of the mutant cells was significantly delayed when the concentration of NaCl was above 0.3 M. At 0.9 M NaCl, wild-type cells recovered with a half time of about 40 min, while mutant cells did not recover. The kinetics of changes in Chi fluorescence confirmed these results. In wild-type cells, input of electrons from the cytosol to PSI via the NAD(P)H-dehy-drogenase complex increased upon salt shock. It appears, therefore, that the electron flow from the cytosol to PSI via NAD(P)H-dehydrogenase is essential for the adaptation of cyanobacteria to salt shock.

Keywords: Cyclic electron transport; NAD(P)H-dehy-drogenase; ndhB gene; Photosystem I; Salt stress; Synechocystis sp. PCC 6803

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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