Journal Article

cDNA Cloning and Tissue Specific Expression of a Gene for Sucrose Transporter from Rice (<i>Oryza sativa</i> L.)

Tatsuro Hirose, Nobuyuki Imaizumi, Graham N. Scofield, Robert T. Furbank and Ryu Ohsugi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 12, pages 1389-1396
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029134
cDNA Cloning and Tissue Specific Expression of a Gene for Sucrose Transporter from Rice (Oryza sativa L.)

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  • Molecular and Cell Biology
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We describe the cloning and expression analysis of a sucrose transporter cDNA from a monocot (the rice plant, Oryza sativa L.). The cDNA clone (OsSUT1) encoded an open reading frame of 1,611 bp (537 amino acids) and showed 76.8 to 79.7% similarity at the amino acid level to other sucrose transporters of dicot species. The predicted membrane topology of OsSUTI protein is made up of 12 transmembrane helices which is consistent with most of the mono- and disaccharide transporters previously identified. When OsSUT1 cDNA was introduced into yeast and expressed, the cells rapidly accumulated sucrose demonstrating that OsSUT1 does, in fact, encode a sucrose transporter. From genomic Southern hybridization OsSUT1 appeared to be a single copy gene. OsSUT1 was expressed in source organs such as leaf blade, leaf sheath and germinating seed whereas little or no expression was observed in some sink organs such as the panicles before heading and the roots. Transcript was observed at high levels in panicles after heading, particularly in the portion containing endosperm and embryo. In addition, expression of OsSUT1 was high in etiolated seedlings and decreased during light-induced greening.

Keywords: Gene expression; Oryza sativa L.; Rice plant; Sucrose phosphate synthase; Sucrose transporter

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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