Journal Article

A Pumpkin 72-kDa Membrane Protein of Precursor-Accumulating Vesicles Has Characteristics of a Vacuolar Sorting Receptor

Tomoo Shimada, Miwa Kuroyanagi, Mikio Nishimura and Ikuko Hara-Nishimura

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 12, pages 1414-1420
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029138
A Pumpkin 72-kDa Membrane Protein of Precursor-Accumulating Vesicles Has Characteristics of a Vacuolar Sorting Receptor

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Precursor-accumulating (PAC) vesicles were previously shown to mediate the transport of the precursor of a major storage protein (pro2S albumin) to protein-storage vacu-oles in developing pumpkin cotyledons. In this study, we characterized two homologous proteins from PAC vesicles, a 72 kDa protein (PV72) and an 82 kDa protein (PV82). PV72 and PV82 showed an ability to bind to peptides derived from both an internal propeptide and a C-terminal peptide of pro2S albumin. PV72 was predicted to be a type I integral membrane protein with epidermal growth factor (EGF)-like motifs. These results suggest that PV72 and PV82 are potential sorting receptors for 2S albumin to protein-storage vacuoles.

Keywords: EGF-like motif; Precursor-accumulating vesicle (PAC vesicle); Pumpkin (Cucurbita sp.); Sorting receptor; Targeting signal; Vacuolar protein

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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