Journal Article

Expression of a Gene for Uricase II (Nodulin-35) in Cotyledons of Soybean Plants

Kenichi Takane, Koji Tanaka, Shigeyuki Tajima, Katsuichiro Okazaki and Hiroshi Kouchi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 2, pages 149-154
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029145
Expression of a Gene for Uricase II (Nodulin-35) in Cotyledons of Soybean Plants

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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A cDNA clone (URcot-35) was isolated from a soybean cotyledonary cDNA library using a cDNA clone (UR-nod-35) for nodule uricase II as a probe. URcot-35 was a 1,170-bp cDNA with an open reading frame that encoded a protein of putative 309 amino acids with a molecular mass of 35,137 Da. The nucleotide sequence of URcot-35 was identical to that of URnod-35.

Expression of the URcot-35 gene in cotyledons was investigated by Northern dot-blot hybridization, by the reverse transcription-polymerase chain reaction with subsequent hybridization assays with the cDNA for nodule uricase II as a probe, and by immunoblotting analysis with a monoclonal antibody that was specific to nodule uricase II. The results suggested that the transcript of URcot-35 was present in developing cotyledons and that uricase II accumulated during the pod-filling stage. This is the first report of the isolation of cDNA for uricase II from non-symbiotic tissue and the results demonstrate that uricase II in soybean cotyledons is identical to that in soybean nodules.

Keywords: Cotyledons; Gene cloning; Gene expression; Nitrogen fixation; Nodules; Uricase

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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