Journal Article

Intracellular pH and Proton-Transport in Barley Root Cells under Salt Stress: in Vivo <sup>31</sup>P-NMR Study

Maki Katsuhara, Yoshiaki Yazaki, Katsuhiro Sakano and Toshio Kawasaki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 2, pages 155-160
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029146
Intracellular pH and Proton-Transport in Barley Root Cells under Salt Stress: in Vivo 31P-NMR Study

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Salt stress-induced changes of intracellular pH and in levels of phosphorous compounds were monitored in intact root tips of barley seedlings (Hordeum vulgare cv. Akashin-riki) by in vivo 31P-nuclear magnetic resonance (NMR) spectroscopy. Vacuolar alkalization was observed after treatment with both 300 and 500 mM NaCl. Much of the observed apparent alkalization of the cytoplasm was eliminated when the effect of Na+ ions on the titration curve was considered. Within 1 h after the initiation of salt stress, levels of glucose-6-phosphate and UDP-glucose decreased markedly, and such decreases might lead directly or indirectly to cell death. Simultaneous measurements of the external and intracellular pH revealed the promotion of external acidification and internal alkalization during salt stress. Possible mechanisms of Na+/H+ antiport at the tonoplast and the role of proton-pump in the plasma membrane are discussed.

Keywords: Barley root; Cytoplasmic pH; In vivo 31P-NMR; Na+/H+ antiport; Salt stress; Vacuolar pH

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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