Journal Article

<i>VR-ACS6</i> is an Auxin-Inducible l-Aminocyclopropane-l-carboxylate Synthase Gene in Mungbean (<i>Vigna radiata</i>)

In Sun Yoon, Hitoshi Mori, Jeong Hoe Kim, Bin G. Kang and Hidemasa Imaseki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 3, pages 217-224
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029156
VR-ACS6 is an Auxin-Inducible l-Aminocyclopropane-l-carboxylate Synthase Gene in Mungbean (Vigna radiata)

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  • Molecular and Cell Biology
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We have isolated four cDNA clones of ACC synthase from etiolated mungbean seedlings treated with auxin. PVR-ACS2, pVR-ACS3 and pVR-ACS6 contained the same sequences as the previously reported DNA fragments, pMAC2, pMAC3 (Botella et al. 1992b) and pMBAl (Kim et al. 1992), respectively. pVR-ACSl was identical with pAIM-1 (Botella et al. 1992a).

VR-ACS6 was specifically induced in response to the auxin signal. The IAA-induction of VR-ACS6 was very rapid (within 30 min) and insensitive to cycloheximide treatment at concentrations up to 100 μM. Significant accumulation of VR-ACS6 mRNA was detected at 1 μM.IAA.The IAA-induced expression of VR-ACS6 was suppressed by ABA and ethylene, but enhanced by BA. These characteristics of VR-ACS6 expression were well correlated with the physiological data of auxin-induced ethylene production in mungbean hypocotyls.

VR-ACS1 was strongly induced by cycloheximide, but was found to be not auxin-specific. Inhibitors of either ethylene biosynthesis (AOA) or action (NBD) increased the basal level of VR-ACS1 mRNA.

Keywords: ACC synthase isogenes; Auxin; Cycloheximide; ifferential expression; Ethylene biosynthesis; Mungbean

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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