Journal Article

Various Pectin Methyltransferase Activities with Affinity for Low and Highly Methylated Pectins

Thierry Bourlard, Annick Schaumann-Gaudinet, Marie-Pierre Bruyant-Vannier and Claudine Morvan

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 3, pages 259-267
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029161
Various Pectin Methyltransferase Activities with Affinity for Low and Highly Methylated Pectins

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The activity of pectin methyltransferases (PMT) from endomembranes of flax cells (Linum usitatissimum L.) was enhanced in the presence of exogenous pectins. The value of optimal pH increased from 5.5 to 7.0 with the degree of methylesterification (DE from 0.00 to 0.50) of pectins. We showed, using size exclusion chromatography, that methylesterification had principally occurred onto exogenous pectins. PMT activity, measured in vitro at pH 7.0 and in the presence of highly methylated pectins, was maximum when tested during the fast growth-phase of cells. In contrast, a major peak occurred at pH 5.5 in the presence of low-methylated pectins over the maturation phase. Two successive sucrose-gradient centrifugations led to the fractiona-tion of low-density membranes (density 1.08) with PMT activity only detected at pH 5.5 and in the presence of low-methylated pectins (DE 0.10). On the other hand, membranes of density 1.12-1.14 were enriched in PMT with a maximum of activity that happened at pH 7.0 and in the presence of highly methylated pectins (DE 0.50). These experiments indicated two types of pectin methyltransferase activities. However, their apparent Km(s) for the donor of methyl, S-adenosyl methionine (about 20 μM), and for the pectic substrate (1 mM galacturonic acid or 0.25 mg ml–1) were similar.

Keywords: Biosynthesis; Cell-wall; Flax; Golgi apparatus; Methyltransferase (EC 2.1.1); Pectin

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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