Journal Article

Conversion of Indole-3-Acetaldehyde to Indole-3-Acetic Acid in Cell-Wall Fraction of Barley (<i>Hordeum vulgare</i>) Seedlings

Ken-ichi Tsurusaki, Kazuyoshi Takeda and Naoki Sakurai

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 3, pages 268-273
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029162
Conversion of Indole-3-Acetaldehyde to Indole-3-Acetic Acid in Cell-Wall Fraction of Barley (Hordeum vulgare) Seedlings

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The cell-wall fraction of barley seedlings was able to oxidize indole-3-acetaldehyde (IAAld) to form IAA, whereas the fraction did not catalyze the conversion of in-dole-3-acetonitrile or indole-3-acetamide to IAA. The activity was lower in a semi-dwarf mutant that had an endogenous IAA level lower than that of the normal isogenic strain [Inouhe et al. (1982) Plant Cell Physiol. 23: 689]. The soluble fraction also contained some activity; the activity was similar in the normal and mutant strains. The optimal pH for the conversion of IAAld to IAA in the cell-wall fraction was 7; that of soluble fraction was 6. The Km value of the cell-wall fraction for IAAld was 5 μM; that of soluble fraction was 31 μM. The activity was not solubi-lized by treatments with 1% Nonidet P-40,1 M NaCI, 3 M LiCl, or 50 mM MgCl2. The oxidation activity was increased by the addition of NAD+. These results suggest that IAAld oxidation activity is bound to cell-wall components and that the lower level of IAA in the mutant probably results from reduced activity of oxidation enzyme bound to cell-wall components.

Keywords: Barley; Biosynthesis; Hordeum vulgare; IAA; Indole-3-acetaldehyde; Indole-3-ethanol

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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