Journal Article

Cloning and Functional Expression of a Novel Geranylgeranyl Pyrophosphate Synthase Gene from <i>Arabidopsis thaliana</i> in <i>Escherichia coli</i>

Fen Zhu, Kengo Suzuki, Kazunori Okada, Katsunori Tanaka, Tsuyoshi Nakagawa, Makoto Kawamukai and Hideyuki Matsuda

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 3, pages 357-361
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029174
Cloning and Functional Expression of a Novel Geranylgeranyl Pyrophosphate Synthase Gene from Arabidopsis thaliana in Escherichia coli

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A gene encoding a novel geranylgeranyl pyrophosphate (GGPP) synthase from Arabidopsis thaliana has been identified and termed GGPS5. The gene has been sequenced and expressed in Escherichia coli. The deduced amino acid sequence showed 64.5% and 57.5% identity with a putative GGPP synthase from Arabidopsis and Capsicum annuum, respectively. GGPP enzymatic activity was detected in E. coli cells expressing the GGPS5 gene in two different ways. One was the direct measurement of GGPP synthase activity in cell extracts and the other was the yellow color production of cells when the GGPS5 gene was co-expressed with crtB, crtI, crtX, crtY and crtZ genes derived from Erwinia uredovora.

Keywords: Arabidopsis thaliana; Carotenoid; crtE; GGPP synthase

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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