Journal Article

Heterologous Expression and Subcellular Localization of Pumpkin Seed Tonoplast Intrinsic Proteins (TIP) in Yeast Cells

Kaori Inoue, Yoh Wada, Mikio Nishimura and Ikuko Hara-Nishimura

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 3, pages 366-370
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029176
Heterologous Expression and Subcellular Localization of Pumpkin Seed Tonoplast Intrinsic Proteins (TIP) in Yeast Cells

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  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Two tonoplast intrinsic proteins (TIP) of pumpkin seeds, pMP23 and MP28, were expressed in yeast cells under control of the GAL1 promoter, and the subcellular localization of the proteins was analyzed. The pMP23 and MP28 stably accumulated in the yeast vacuolar membrane when the proteins were expressed in the proteinase A-deficient strain (pep4), which lacks the activities of vacuolar proteases. However, pMP23 and MP28 did not accumulate in the wild-type strain; the expressed pMP23 and MP28 were degraded in a proteinase A-dependent manner. These results indicate that pMP23 and MP28 are transported to the vacuolar membrane when expressed in yeast.

Keywords: Protein body; Protein-storage vacuole; Pumpkin; TIP; Vacuole; Yeast

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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