Journal Article

Rapid Reconstitution of Tonoplast H<sup>+</sup>-Translocating Pyrophosphatase from Cultured Rice Cells into Liposomes

Yoshiyuki Nakamura, Yoshikiyo Sakakibara, Hideyuki Kobayashi and Kunihiro Kasamo

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 3, pages 371-374
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029177
Rapid Reconstitution of Tonoplast H+-Translocating Pyrophosphatase from Cultured Rice Cells into Liposomes

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We report the rapid and functional reconstitution of H+-pyrophosphatase (H+-PPase) from the tonoplast of cultured rice (Oryza sativa L.) cells to proteoliposomes. The CHAPS-solubilized H+-PPase was incorporated into liposomes by gel-filtration. Both the activities of PPi-hy-drolysis and H+-pumping were influenced by the lipid-pro-tein ratio and cholesterol.

Keywords: CHAPS; Gel-filtration; H+-pumping; H+-PPase reconstitution; Rice (Oryza sativa)

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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