Journal Article

Herbicide Safener-Inducible Gene Expression in <i>Arabidopsis thaliana</i>

Lieven de Veylder, Marc van Montagu and Dirk Inzé

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 5, pages 568-577
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029206
Herbicide Safener-Inducible Gene Expression in Arabidopsis thaliana

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The potential use of a new chemical-inducibie gene expression system in Arabidopsis thaliana has been examined. The system is based on the maize In2-2 promoter which is activated by benzenesulfonamide herbicide safe-ners. Plants transformed with the β-glucuronidase (gus) reporter gene under the control of the In2-2 promoter were grown in the presence of different safeners and the induced GUS activity pattern was studied histochemically. In the absence of safeners, the In2-2 promoter was not active. Application of different safeners induced distinct gus expression patterns, including expression in the root, hy-dathodes, and the shoot apical meristem. Plants maintained continuously on inducing concentrations of the safeners were retarded in growth. The growth inhibition effects of the Sa5 safener could be overcome in a sul-fonylurea-resistant background. In2-2 promoter activity could also be induced by the sulfonylurea herbicide chlor-sulfuron. In the sulfonylurea-resistant background, which derives from herbicide-resistant acetolactate synthase activity, induction of the In2-2 promoter by chlorsulfuron was lower. Furthermore, branched-chain amino acids, known to inhibit acetolactate synthase activity, also induced In2-2 promoter activity. Our data suggest a strong correlation between In2-2 expression and inhibition of the acetolactate synthase activity.

Keywords: Acetolactate synthase; Arabidopsis thaliana; Chlorsulfuron; Safeners

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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