Journal Article

Isolation and Characterization of cDNA Clones with Enhanced Expression in Tobacco Plants Expressing the Rice Homeobox Gene, <i>OSH1</i>

Masanori Tamaoki, Naoki Yamamoto and Makoto Matsuoka

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 5, pages 638-642
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029215
Isolation and Characterization of cDNA Clones with Enhanced Expression in Tobacco Plants Expressing the Rice Homeobox Gene, OSH1

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We have used subtractive hybridization to isolate cDNA clones whose expression were up-regulated in transgenic tobacco ectopically expressing the rice homeobox gene, OSH1. Thirty-nine distinct cDNA clones, which we term HRGs (Homeobox Regulated Genes), were identified. Some of them were specifically expressed in transformants, indicating that their expression was possibly regulated by transgene.

Keywords: cDNA subtraction; Nicotiana tabacum; Rice homeobox gene; Transgenic tobacco

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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