Journal Article

Purification and Characterization of Tobacco Pathogenesis-Related Protein PR-5d, an Antifungal Thaumatin-like Protein

Hisashi Koiwa, Hiroaki Kato, Toru Nakatsu, Junichi Oda, Yasuyuki Yamada and Fumihiko Sato

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 7, pages 783-791
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029236
Purification and Characterization of Tobacco Pathogenesis-Related Protein PR-5d, an Antifungal Thaumatin-like Protein

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  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Cultured tobacco cells accumulate several pathogene-sis-related proteins. A neutral PR-5 protein, PR-5d, was purified to homogeneity from such cells. PR-5d has highly hy-drophobic characteristics, but hydropathy analysis of its primary structure did not show a hydrophobic domain. In a series of bioassays, purified PR-5d showed inhibitory activity against several phytopathogenic and non-phytopatho-genic fungi as do other members of the PR-5 protein family. To study the antifungal mechanism based on three dimensional structure of PR-5d, purified PR-5d was crystallized. The preliminary X-ray analysis of the crystal revealed that the crystals belong to space group C2, with cell dimensions a=80.2 Å, b=63.8 Å, c=45.7 Å, and β= 107.2°, and diffract at least 1.8 Å resolution.

Keywords: Antifungal activity; Crystallization; Nico-tiana tabacum cv. Samsun NN; Osmotin; Pathogenesis-related protein PR-5d; Thaumatin

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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