Journal Article

The <i>Spirulina platensis</i> Adenylate Cyclase Gene, <i>cyaC</i>, Encodes a Novel Signal Transduction Protein

Masahiro Kasahara, Kumiko Yashiro, Toshio Sakamoto and Masayuki Ohmori

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 7, pages 828-836
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029241
The Spirulina platensis Adenylate Cyclase Gene, cyaC, Encodes a Novel Signal Transduction Protein

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

A cyaC gene encoding an adenylate cyclase of the filamentous cyanobacterium Spirulina platensis was se-quenced. The predicted amino acid sequence of the C-ter-minal region of cyaC is similar to the catalytic domains of adenylate cyclases in other cyanobacteria and eukaryotes. The sequences of other regions are similar to those of proteins consisting of the bacterial two-component signal transduction system: the sensory kinase and the response regulator. The predicted gene product of cyaC contains, from the N-terminal end, a receiver domain of the response regulator protein (Rl), a domain similar to the ETR1 of Arabi-dopsis thaliana, a transmitter domain of the sensory kinase protein, a receiver domain of the response regulator protein (R2), and a catalytic domain of adenylate cyclase. The cyaC gene was expressed as an affinity-tagged protein in Escherichia coli, and the recombinant protein was purified. The purified protein had adenylate cyclase activity which was activated by Mn2+. The results of Western blotting using an anti-CyaC antiserum and the S. platensis cell extract confirmed that cyaC gene is expressed in S. platensis

Keywords: Adenylate cyclase (EC 4.6.1.1); Bacterial two-component regulatory system; Cyanobacterium; Response regulator; Sensory kinase

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.