Journal Article

Membrane Depolarization Induced by <i>N</i>-Acetylchitooligosaccharide Elicitor in Suspension-Cultured Rice Cells

Munehiro Kikuyama, Kazuyuki Kuchitsu and Naoto Shibuya

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 8, pages 902-909
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029250
Membrane Depolarization Induced by N-Acetylchitooligosaccharide Elicitor in Suspension-Cultured Rice Cells

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N-acetylchitooIigosaccharides (fragments of chitin) elicit defense responses, including phytoalexin production, in suspension-cultured rice cells. They induced rapid and transient membrane depolarization accompanied by a transient increase in net CP-efflux. The membrane depolarization was not affected by anaerobiosis or azide, suggesting that the major part of the depolarization was mediated by ion channels, not by energy-dependent ion pumps. Depolarization was partly inhibited in the presence of Ca2+- or Cl-channel blockers and highly inhibited by depletion of Ca2+ in the extracellular medium. A calcium ionophore, A23187, caused a transient depolarization but not an increase in Cl efflux, while it did not inhibit the elic-itor-induced transient depolarization and Cl efflux. These suggest that the influx of Ca2+ from the extracellular space to the cytoplasm is necessary as an initial trigger but not sufficient for membrane depolarization, Cl efflux, and the following signaling processes.

Keywords: Ca2+; Defense response; Elicitor; Membrane potential; Signal transduction; Suspension-cultured rice cell

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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