Journal Article

Lipids of <i>Ectocarpus fasciculatus</i> (Phaeophyceae). Incorporation of [l-<sup>14</sup>C]Oleate and the Role of TAG and MGDG in Lipid Metabolism

Anja Makewicz, Corinne Gribi and Waldemar Eichenberger

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 8, pages 952-962
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029257
Lipids of Ectocarpus fasciculatus (Phaeophyceae). Incorporation of [l-14C]Oleate and the Role of TAG and MGDG in Lipid Metabolism

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Lipids and fatty acids of Ectocarpus fasciculatus (Ectocarpales, Phaeophyceae) were analyzed. Major polar lipids are monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), sulfoquinovosyldiacylglycerol (SQDG), diacylglycerylhydroxymethyl-N,N,N-trimethyl-rβ-alanine (DGTA), phosphatidylcholine (PC), phospha-tidylethanolamine (PE), phosphatidylglycerol (PG) and phosphatidylinositol (PI). Diphosphatidylglycerol (DPG), phosphatidic acid (PA) and phosphatidyl-O-[N-(2-hydroxy-ethyl)glycine] (PHEG) were also present in small amounts. Nonpolar lipids mainly consist of triacylglycerol (TAG) and diacylglycerol (DAG). Major fatty acids are 16:0,18:1, α18:3, 18:4, 20:4 and 20:5. The positional distribution of fatty acids showed that molecular species of eukaryotic structure account for 99% in MGDG, 98% in DGDG, 62% in PG and 23% in SQDG. On incubation with [1-14C]18:1 for 30 min, 33% of the total label was detected in TAG, 16% in PG, 14% in PE, 10% in PC and 8% in MGDG. During 7 days of chase, the label in TAG, PG, PE and PC decreased and simultaneously increased in MGDG up to 41% of the total. In SQDG, labelled fatty acids were found in prokaryotic as well as in eukaryotic molecular species. During the experiment, the label shifted from 18:1 to 18:2, 18:3, 18:4 and, to a minor extent, to 20:4 and 20:5 acids indicating 18:1 to be processed by elongation and/or desaturation. These results suggest TAG to act as a major primary acceptor of exogenous oleate and to be involved in the transfer of fatty acids to MGDG and other polar lipids.

Keywords: Ectocarpus fasciculatus; Fatty acids; Lipid metabolism; Monogalactosyldiacylglycerol; Phaeophyceae; Triacylglycerol

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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