Journal Article

Differential Expression of Two Genes for Sucrose-Phosphate Synthase in Sugarcane: Molecular Cloning of the cDNAs and Comparative Analysis of Gene Expression

Bambang Sugiharto, Hitoshi Sakakibara, Sumadi and Tatsuo Sugiyama

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 8, pages 961-965
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029258
Differential Expression of Two Genes for Sucrose-Phosphate Synthase in Sugarcane: Molecular Cloning of the cDNAs and Comparative Analysis of Gene Expression

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Two cDNA clones, pSoSPSl and pSoSPS2, encoding sucrose-phosphate synthase (SPS) of sugarcane were isolated from a leaf cDNA library. Northern analysis revealed the transcript of SoSPSl to be predominant in leaves, but that of SoSPS2 to be distributed not only in leaves but also in roots at a similar level. The transcript of SoSPSl was markedly accumulated during greening of etiolated leaves, whereas that of SoSPS2 was constitutively expressed. These findings indicate that SPS in sugarcane is encoded by multiple genes, which show organ-specificity and are differentially regulated in response to light.

Keywords: cDNA cloning; Gene expression; Sucrose-phosphate synthase (EC 2.4.1.14); Sugarcane

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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