Journal Article

Internal Gln/Glu Ratio as a Potential Regulatory Parameter for the Expression of a Cytosolic Glutamine Synthetase Gene of Radish in Cultured Cells

Akio Watanabe, Noriko Takagi, Hiroaki Hayashi, Mitsuo Chino and Akira Watanabe

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 9, pages 1000-1026
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029263
Internal Gln/Glu Ratio as a Potential Regulatory Parameter for the Expression of a Cytosolic Glutamine Synthetase Gene of Radish in Cultured Cells

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The radish Glnl;l gene encodes cytosolic glutamine synthetase, and is strongly expressed during senescence of cotyledons [Watanabe et al. (1994) Plant Mol. Biol. 26: 1807]. We showed previously that nitrogenous compounds exogenously applied to the cotyledons significantly affected the transcript level of the gene during senescence, suggesting that the gene is possibly under metabolic control by cellular nitrogen status. To investigate such metabolic regulation of the gene, we tested the response of the gene to the changes in cellular nitrogen status, using radish cultured cells. Northern blotting and measurement of free amino acids in the cells showed that the radish cells accumulating glutamine at high levels exhibited contrasting low levels of Glnl;l transcripts. Further and detailed study provided evidences suggesting that the transcript level was regulated in response to the ratio of cellular glutamine to glutamate, but not to the glutamine level alone.

Keywords: Cultured cells; Gene expression; Glutamine synthetase (EC 6.3.1.2); Nitrogen metabolism; Raphanus sativus

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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