Journal Article

Isolation and Characterization of Matrix Associated Region DNA Fragments in Rice (<i>Oryza sativa</i> L.)

Koji Nomura, Wataru Saito, Kimiyo Ono, Hiromitsu Moriyama, Seiko Takahashi, Masayasu Inoue and Kiyoshi Masuda

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 38, issue 9, pages 1060-1068
Published in print January 1997 | ISSN: 0032-0781
Published online January 1997 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029272
Isolation and Characterization of Matrix Associated Region DNA Fragments in Rice (Oryza sativa L.)

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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To investigate the interactions between chromosomal DNA and nuclear matrices in higher plants, matrix associated regions (MARs) of rice (Oryza sativa L.) DNAs were cloned. First, we prepared nuclear matrices from isolated nuclei by digesting them with EcoRl and then extracting with 2 M NaCl. About 6% of the total DNA remained in the nuclear matrices after this digestion and extraction. The residual DNA fragments in the nuclear matrices were cloned. Some of the cloned DNA fragments showed binding to certain nuclear proteins. One of the MAR fragments contained sequences related to known consensus motifs and a hairpin loop structure. A method is presented for isolation of matrix associated region (MAR) DNAs from plant cells.

Keywords: Cell culture; Matrix associated region; Nuclei; Nuclear matrices; Rice; Oryza sativa L.

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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