Journal Article

Evidence for the Cell Wall Involvement in Temporal Changes in Freezing Tolerance of Jerusalem Artichoke <i>{Helianthus tuberosus</i> L.) Tubers during Cold Acclimation<sup>1</sup>

Mari Murai and Sbizuo Yoshida

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 1, pages 97-105
Published in print January 1998 | ISSN: 0032-0781
Published online January 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029295
Evidence for the Cell Wall Involvement in Temporal Changes in Freezing Tolerance of Jerusalem Artichoke {Helianthus tuberosus L.) Tubers during Cold Acclimation1

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We studied the mechanism of cold acclimation of Jerusalem artichoke {Helianthus tuberosus L.) tubers with special reference to the role of the cell wall. During the cold-acclimation process from September to January, the freezing tolerance of tubers increased from − 2.8°C to −8.4°C (LT50). By contrast, the isolated protoplasts con- stitutively showed a consistent high level of freezing toler ance (LT50; below − 25°C) throughout the period. In tuber tissues, freezing injury was effectively protected by the ex ternal addition of isotonic solutions. Cryomicroscopic ob servations revealed that tissue cells mounted in isotonic so lutions plasmolyzed upon freezing; tissue cells mounted in water collapsed with a tight attachment of plasma mem brane to the cell wall. Upon freezing of intact tissues in water to temperatures below the critical range, the cyto plasm was irreversibly acidified as revealed by a fluores cence pH-ratiometry, suggesting that occurrence of detri mental cellular events leading to permanent cell injury. The freeze-induced acidification of cytoplasm was also effective ly prevented by the external addition of isotonic solutions. These results suggest that the tight attachment of the plas ma membrane to the cell wall during freezing may have a harmful effect on cells, in particular on the plasma mem brane, possibly due to mechanical or some sort of chemi cal/physico-chemical interaction with the cell wall.

Keywords: Cell wall; Cytoplasmic pH; Freezing injury; Jerusalem artichoke; Protoplast

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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