Journal Article

Localization of the Xyloglucan in Cell Walls in a Suspension Culture of Tobacco by Rapid-Freezing and Deep-Etching Techniques Coupled with Immunogold Labelling

Kiyoshi Suzuki, Kei'ichi Baba, Takao Itoh and Yosbiaki Sone

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 10, pages 1003-1009
Published in print October 1998 | ISSN: 0032-0781
Published online October 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029296
Localization of the Xyloglucan in Cell Walls in a Suspension Culture of Tobacco by Rapid-Freezing and Deep-Etching Techniques Coupled with Immunogold Labelling

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Localization of xyloglucan in cell walls regenerated from tobacco protoplasts (Nicotiana tabacum L.; cv. BY-2) is visualized by rapid-freezing and deep-etching (RFDE) electron microscopy coupled with immunogold electron microscopy. Xyloglucan was already deposited in the cell wall 3 h after culture initiation. Xyloglucan was mainly localized along microfibrils with a lesser amount in intersections between two crossed microfibrils in 120-hour-old cells. These data support the previous hypothesis of Keegstra et al. (1973) that propose an interconnection between xyloglucan and cellulose.

Keywords: Deep-etching; Immunogold labelling; Nicotiana tabacum L.; Protoplasts; Xyloglucan

Journal Article.  0 words. 

Subjects: Plant Sciences and Forestry ; Biochemistry ; Molecular and Cell Biology

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