Journal Article

Changes in Protein Interactions of Cell Cycle-Related Genes during the Dormancy-to-Growth Transition in Pea Axillary Buds

Sae Shimizu and Hitoshi Mori

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 10, pages 1073-1079
Published in print October 1998 | ISSN: 0032-0781
Published online October 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029304
Changes in Protein Interactions of Cell Cycle-Related Genes during the Dormancy-to-Growth Transition in Pea Axillary Buds

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  • Plant Sciences and Forestry
  • Biochemistry
  • Molecular and Cell Biology

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Apical dominance is a phenomenon in which a terminal bud grows predominantly and the growth of the axillary buds is suppressed. Here, we investigated the molecular mechanisms associated with cell cycle control that occur in pea axillary buds as a result of decapitation. Proliferating cell nuclear antigen (PCNA) protein was detected in both dormant and growing buds, while PCNA mRNA was absent in dormant buds. Pissa;CycBl;2 and Cdc2 proteins were undetectable during dormancy. To analyze an interaction between PCNA and Pissa;CycD3;l, we performed anti-PCNA immunoaffinity column chromatography. Pissa;CycD3;l protein was detected in the eluate prepared from the dormant buds, but not in the eluate prepared from the growing buds. Furthermore, we performed anti-Pissa;CycD3;l immunoaffinity column chromatography. PCNA protein was detected in the eluate prepared from the dormant buds, but not in the eluate prepared from the growing buds. These results indicated that PCNA associated with Pissa;CycD3;l only during dormancy. In addition, the interaction between PCNA and Pissa;CycD3;l was confirmed by a yeast two-hybrid system.

Keywords: Apical dominance; Dormancy; Immunoaffinity column; Pissa;CycD3;l; Proliferating cell nuclear antigen (PCNA); Pisum sativum.

Journal Article.  0 words. 

Subjects: Plant Sciences and Forestry ; Biochemistry ; Molecular and Cell Biology

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