Journal Article

Effect of Mastoparan on Phospholipase A<sub>2</sub> Activity in Potato Tubers Treated with Fungal Elicitor

Kaori Senda, Noriyuki Doke and Kazuhito Kawakita

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 10, pages 1080-1098
Published in print October 1998 | ISSN: 0032-0781
Published online October 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029305
Effect of Mastoparan on Phospholipase A2 Activity in Potato Tubers Treated with Fungal Elicitor

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  • Biochemistry
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Participation of phospholipase (PL) A2 in signal trans-duction has been reported to elicitate a resistance reaction in potato cells by inoculation of an incompatible race of Phytophthora infestans, the late blight fungus, or by treatment with fungal elicitor hyphal wall components (HWC). Mastoparan, a generic G protein activator, has been shown to activate PLA in a G protein-dependent manner in animal cells. We analyzed the effects of mastoparan and the inactive analog Mas-17 on PLA2 activity in potato tubers. In healthy potato tubers, the activation of PLA2 by mastoparan was detected in the soluble fraction, but not micro-somal fraction. However, in potato tubers treated with HWC, PLA2 activity was stimulated by mastoparan in both soluble and microsomal fractions. Pretreatment of the microsomal fraction with neomycin, a PLC inhibitor, and staurosporine, a protein kinase inhibitor, inhibited the mastoparan-induced activation of PLA2. This suggested that the PLA2 activation in potato tubers by mastoparan was mediated by the PLC pathway and protein phospho-rylation. We also examined the accumulation of potato phytoalexin rishitin. Mastoparan stimulated rishitin accumulation induced by HWC, but did not induce the accumulation. This indicated that mastoparan might activate the signal transduction pathway in the resistance reactions induced in potato tubers.

Keywords: G protein; Mastoparan; Phospholipase A2(EC 3.1.1.4); Potato tuber; Resistance reaction; Signal transduction

Journal Article.  0 words. 

Subjects: Plant Sciences and Forestry ; Biochemistry ; Molecular and Cell Biology

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