Journal Article

Bacteroids Isolated from Ineffective Nodules of <i>Pisum sativum</i> Mutant E135 (<i>sym</i>l3) Lack Nitrogenase Activity but Contain the Two Protein Components of Nitrogenase

Norio Suganuma, Nobutaka Sonoda, Chizuru Nakane, Kenji Hayashi, Takamitsu Hayashi, Masanori Tamaoki and Hiroshi Kouchi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 10, pages 1093-1098
Published in print October 1998 | ISSN: 0032-0781
Published online October 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029307
Bacteroids Isolated from Ineffective Nodules of Pisum sativum Mutant E135 (syml3) Lack Nitrogenase Activity but Contain the Two Protein Components of Nitrogenase

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Pea mutant E135 (sym15) forms ineffective (Fix) nodules that lack nitrogen fixing activity. To determine the developmental step blocked in E135 nodules we studied the nitrogenase activities in isolated bacteroids and in cell-free extracts of bacteroids, and measured the two components of nitrogenase protein in bacteroids. Bacteroids prepared anaerobically from E135 nodules showed no acetylene reduction activity in the presence and absence of myoglobin. Furthermore, no acetylene reduction activity by cell-free extracts of E135 bacteroids was detected in the presence of ATP-generating system and dithionite. However, immuno-blotting analyses revealed the presence of nitrogenase components I and II in E135 nodule bacteroids. These results suggest that a host plant gene is involved in the expression of nitrogenase activity in symbiotic bacteria.

Keywords: Bacteroids; Ineffective nodules; Mutant; Nitrogenase; Pisum sativum; Symbiotic gene

Journal Article.  0 words. 

Subjects: Plant Sciences and Forestry ; Biochemistry ; Molecular and Cell Biology

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