Journal Article

Effect of Ions on the Orientation of Cortical Microtubules in <i>Spirogyra</i> Cells

Kazuyoshi Iwata and Takao Itoh

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 10, pages 1099-1103
Published in print October 1998 | ISSN: 0032-0781
Published online October 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029308
Effect of Ions on the Orientation of Cortical Microtubules in Spirogyra Cells

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Effects of ions on the orientation of cortical micro-lubules (MTs) in Spirogyra cells were studied. After depo-lymerizalion with amiprophos-methyl (APM), MTs were allowed to reorganize in NaCI solutions of various concentrations. As the concentration of NaCI increased, the frequency of cells that had oblique MTs increased. When cells in NaCI solution were transferred into artificial pond water (APW) and incubated for 6 h, all the MTs changed to become transverse to the longitudinal axis of the cell. KC1 and MgCl2 also had effects on the orientation of MTs. However, NH4Cl, CaCl2;, CoCl2, and Co(NO3)2 did not show any effect. These results suggest that Na+, K+, and Mg2+have effects on MT orientation and that NH+4, Ca2+, Co2+, Cl, and NO3 have little effect. When MTs were reorganized in either NaCl or KCl solutions, all the oblique MTs were organized into an S-helix. In contrast, some of the oblique MTs were found as a Z-helix in the cells incubated in MgCl2 or mannitol solutions. These results suggest that effects of Na+ and K+ on the orientation of MTs are not the same as those of Mg2+ and mannitol. These results provide the first evidence that ions are involved in the orientation of MTs in algae.

Keywords: Amiprophos-methyl; Cortical microtubule; Ion; Spirogyra

Journal Article.  0 words. 

Subjects: Plant Sciences and Forestry ; Biochemistry ; Molecular and Cell Biology

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