Journal Article

Isolation and Characterization of a cDNA Clone of UDP-Galactose: Flavonoid 3-<i>0</i>-Galactosyltransferase (UF3GaT) Expressed in <i>Vigna mungo</i> Seedlings

Masami Mato, Yoshihiro Ozeki, Yoshio Itoh, Daisuke Higeta, Kunijiro Yoshitama, Susumu Teramoto, Ryutaro Aida, Nariyuki Ishikura and Michio Shibata

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 11, pages 1145-1155
Published in print November 1998 | ISSN: 0032-0781
Published online November 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029315
Isolation and Characterization of a cDNA Clone of UDP-Galactose: Flavonoid 3-0-Galactosyltransferase (UF3GaT) Expressed in Vigna mungo Seedlings

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Four cDNA clones were isolated from Vigna mungo seedlings by the screening with cDNA encoding UDP-glu-cose:flavonoid 3-0-glucosyltransferase (UF3GT) of Antirrhinum majus as a probe; the product of the gene corresponding to one cDNA was more highly expressed in the first simple leaves than in stems. Nucleotide sequence analysis revealed 1,691 bp (including 326 bp non-reading) containing an open reading frame of 455 amino acids. The deduced amino acid sequence showed 42% and 23% identity with those of A. majus UDP-glucose:flavonoid 3-O-glucosyltransferase (UF3GT) and Petunia hybrida UDP-rhamnose:anthocyanidin 3-0-glucoside rhamnosyltrans-ferase (RT), respectively. One region of the cDNA (amino acids 325 to 387) showed similarity to ceramide UDP-galac-tosyltransferases of mice, rats and humans. A crude extract from Escherichia coli, in which the protein was expressed from the cDNA, showed high UF3GaT activity but low UF3GT activity, and was similar in Km, optimal pH and substrate specificity to UF3GaT from V. mungo. We conclude that we have obtained UDP-galactose:flavonoid 3-0-galactosyltransferase (UF3GaT) cDNA from V. mungo.

Keywords: Black gram; Cloning; Flavonoid; Gene expression; UDP-galactose:flavonoid 3-O-galactosyl-transferase (UF3GaT) (EC 2.4.l.X); Vigna mungo

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.